a laboratory manual Parys, Jan B. Bootman, Martin D. Yule, David I. Bultynck, Geert text monographic xvi, 592 pages : illustrations (some color) ; 28 cm "This laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principles and applications of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imaging modalities to characterize Ca2+ signals. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and methods to investigate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and intercellular signals are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice"-- Calcium Calcium Channels Calcium Signaling Fluorometry 572 INS 1990 C161 Or. 9781621820789 (hbk.) 1621820785 (hbk.) 9781936113583 (pbk.) 1936113589 (pbk.)