TY  - BOOK
AU  - Parys, Jan B.,
AU  - Bootman, Martin D.,
AU  - Yule, David I.,
AU  - Bultynck, Geert,
TI  - Calcium techniques: a laboratory manual 
SN  - 9781621820
U1  - 572 INS 1990 C161 Or. 
KW  - Calcium
KW  - Calcium Channels
KW  - Calcium Signaling
KW  - Fluorometry
N2  - "This laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principles and applications of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imaging modalities to characterize Ca2+ signals. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and methods to investigate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and intercellular signals are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice"--
ER  -