000			01482cam a2200253 i 4500
020			_a9781621820789 (hbk.)
020			_a1621820785 (hbk.)
020			_a9781936113583 (pbk.)
020			_a1936113589 (pbk.)
040			_cDLC
082	0	0	_a572 INS 1990 C161 Or.
245	0	0	_aCalcium techniques : _ba laboratory manual /
300			_axvi, 592 pages : _billustrations (some color) ; _c28 cm
520			_a"This laboratory manual provides step-by-step protocols for studying many facets of Ca2+ signaling, as well as background information on the principles and applications of the techniques. Contributors discuss how to use fluorescent, luminescent, and genetically encoded Ca2+ probes in conjunction with state-of-the-art imaging modalities to characterize Ca2+ signals. Electrophysiological measurements of Ca2+ channel activity are described, as are radioactive Ca2+ flux assays and methods to investigate signaling mediated by specific Ca2+-mobilizing messengers (IP3, cADPR, and NAADP). Techniques to modulate and suppress intra- and intercellular signals are also provided. Each protocol is complete with a list of required materials, detailed recipes for media and reagents, and troubleshooting advice"--
650	1	2	_aCalcium
650	2	2	_aCalcium Channels
650	2	2	_aCalcium Signaling
650	2	2	_aFluorometry
700	1		_aParys, Jan B.,
700	1		_aBootman, Martin D.,
700	1		_aYule, David I.,
700	1		_aBultynck, Geert,
942			_cAEDBB
999			_c19121 _d19121